Parent Category: Laboratoires Published: Thursday, 16 February 2012

Regulation of gene expression

 

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 Dom HELMLINGER

 

 CRBM - UMR 5237

 1919, route de Mende, 34293 Montpellier

 

Office Phone: +33 4 34 35 95 48

Lab Phone: +33 4 34 35 95 50

Email: dhelmlinger@crbm.cnrs.fr

 

Website

 

 

Our lab is specifically interested in understanding how cells respond to developmental or environmental changes by altering gene expression. One critical level of regulation is transcription, which is controlled by many large multiprotein complexes, including coactivators. Coactivator complexes carry multiple distinct activities that play crucial roles in fundamental processes and their perturbation can cause oncogenesis or neurodegeneration. Our objective is to understand how these activities integrate external signals to regulate transcription.

To do this, we focus our studies on one such coactivator, the SAGA complex, and use the fission yeast S. pombe as our model organism. We combines genetic, genomic and proteomic approaches to address three questions: 1) How does SAGA sense and integrate external signals? 2) What are the regulatory roles of SAGA at the genes it controls? 3) Does SAGA regulate gene expression beyond its roles in transcription? We hope to discover new mechanisms by which signal transduction pathways control gene expression in eukaryotes. Given the high conservation of all these factors between yeast and mammals, what we learn in yeast will likely be relevant to similar fundamental aspects of gene expression in humans and how they are perturbed in diseases.

 

Keywords: transcription, chromatin, signaling, yeast

 

 

Main publications

  • Elías-Villalobos A, Toullec D, Faux C, Séveno M, Helmlinger D (2019) Chaperone-mediated ordered assembly of the SAGA and NuA4 transcription co-activator complexes in yeast. Nature Communications 10(1): 5237. doi:10.1038/s41467-019-13243-w.
  • Elías-Villalobos A., Fort P., and Helmlinger D. (2019) New insights into the evolutionary conservation of Tra1, the only pseudoPIKK. Biochemical Society Symposia Nov. 16. DOI: 10.1042/BST20180496.
  • Laboucarié T, Detilleux D, Rodriguez-Mias RA, Faux C, Romeo Y, Franz-Wachtel M, Krug K, Maček B, Villén J, Petersen J, Helmlinger D (2017) TORC1 and TORC2 converge to regulate the SAGA co-activator in response to nutrient availability. EMBO Reports (12):2197-2218.
  • Helmlinger D* and Tora L* (2017) Sharing the SAGA. Trends in Biochemical Sciences 42(11):850-861. *Co-corresponding authors.
  • Elías-Villalobos A, Fernández-Álvarez A, Moreno-Sánchez I, Helmlinger D*, Ibeas JI* (2015) The Hos2 histone deacetylase controls Ustilago maydis virulence through direct regulation of mating-type genes. PLOS Pathogens 11(8): e1005134. *Joint senior authors.
  • Helmlinger D (2012) New insights into the SAGA coactivator complex from its study in S. pombe. Transcription. (1):13-18. Review.
  • Helmlinger D., Marguerat S., Villén J., Swaney DL., Gygi SP., Bähler J., and Winston F. (2011) Tra1 has specific regulatory roles, rather than global functions, within the SAGA coactivator complex. The EMBO Journal 30(14):2843-52.
  • Helmlinger D., Marguerat S., Villén J., Gygi SP., Bähler J., and Winston F. (2008) The S. pombe SAGA complex control the switch from proliferation to sexual differentiation through the opposing roles of its subunits Gcn5 and Spt8. Genes & Development 22(22):3184-95.

 

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