mRNA Regulation and Development
Martine SIMONELIG
IGH - UMR 9004
141, rue de la Cardonille, 34396 Montpellier
Phone: +33 4 34 35 99 59
Email: martine.simonelig@igh.cnrs.fr
Our group is interested in mRNA regulations during development. We are studying regulations of mRNA poly(A) tail length by polyadenylation and deadenylation and the role of these reactions in the control of gene expression during development and in a pathology called oculopharyngeal muscular dystrophy (OPMD). This pathology results from mutations in a protein required for mRNA polyadenylation (Poly(A) Binding Protein Nuclear 1: PABPN1). We are using Drosophila as a model system, a powerful model for in vivo studies.
Our recent data established the key role of deadenylation in the translational control of mRNAs essential for development, including the mRNA encoding the embryonic posterior morphogen Nanos. We have identified the implication of the piRNA pathway in this regulated deadenylation. piRNAs form a specific class of small non-coding RNAs which are produced from transposable elements. Therefore, our results allow to propose for the first time a direct function of transposable elements in development. We are also studying these regulations in germline stem cells as a model of adult stem cell biology.
Keywords: Early development / Germline / mRNA Regulation / RNA silencing / Stem cells
Main publications
- Rojas-Ríos Patricia*, Chartier Aymeric*, Pierson Stéphanie, Séverac Dany, Dantec Christelle, Busseau Isabelle and Simonelig Martine. Translational control of autophagy by Orb in the Drosophila germline. (2015) Developmental Cell, 35, 622-631.
- Barckmann Bridlin*, Pierson Stéphanie*, Dufourt Jérémy*, Papin Catherine, Armenise Claudia, Port Fillip, Grentzinger Thomas, Chambeyron Séverine, Baronian Gregory, Desvignes Jean-Pierre, Curk Tomaz, Simonelig Martine. Aubergine iCLIP reveals piRNA-dependent decay of mRNAs involved in germ cell development in the early embryo. (2015) Cell Reports,12, 1205-16.
- Chartier Aymeric, Klein Pierre, Pierson Stéphanie, Barbezier Nicolas, Gidaro Teresa, Casas François, Carberry Steven, Dowling Paul, Maynadier Laurie, Bellec Maëlle, Oloko Martine, Jardel Claude, Moritz Bodo, Dickson George, Mouly Vincent, Ohlendieck Kay, Butler-Browne Gillian, Trollet Capucine*, Simonelig Martine*. Mitochondrial dysfunction reveals the role of mRNA poly(A) tail regulation in oculopharyngeal muscular dystrophy pathogenesis. (2015) PLoS Genet.,11(3):e1005092.
- Temme Claudia, Simonelig Martine, Wahle Elmar. Deadenylation of mRNA by the CCR4-NOT complex in Drosophila: molecular and developmental aspects. (2014) Front Genet. 5, 143.
- Joly Willy, Chartier Aymeric, Rojas-Rios Patricia, Busseau Isabelle, Simonelig Martine. The CCR4 deadenylase acts with Nanos and Pumilio in the fine-tuning of Mei-P26 expression to promote germline stem cell self-renewal. (2013) Stem Cell Reports, 1, 411-424.
- Barckmann Bridlin, Simonelig Martine. Control of maternal mRNA stability in germ cells and early embryos. Special Issue on RNA decay mechanisms. (2013) BBA-Gene Regulatory Mechanisms, 1829, 714-724.
- Chartier Aymeric, Simonelig Martine. Animal models in therapeutic drug discovery for oculopharyngeal muscular dystrophy. (2012) Drug Discovery Today:Technologies, doi.org/ 10.1016 /j.ddtec.2012.07.002.
- Simonelig Martine. Developmental functions of piRNAs and transposable elements: A Drosophila point-of-view. (2011) RNA Biology, 8:5.
- Barbezier Nicolas, Chartier Aymeric, Bidet Yannick , Buttstedt Anja, Voisset Cécile, Galons Hervé , Blondel Marc, Schwarz Elisabeth and Simonelig Martine. Antiprion drugs 6-aminophenanthridine and guanabenz reduce PABPN1 toxicity and aggregation in oculopharyngeal muscular dystrophy. (2011) EMBO Molecular Medicine, 3, 35-49.
- Rouget Christel, Papin Catherine, Boureux Anthony, Meunier Anne-Cécile, Franco Bénédicte, Robine Nicolas, Lai Eric C., Pélisson Alain, Simonelig Martine. Maternal mRNA deadenylation and decay by the piRNA pathway in the early Drosophila embryo. (2010) Nature, 467, 1128-1132.
