Parent Category: Laboratoires Published: Monday, 05 December 2016

 Viral Trafficking, Restriction and Innate Signaling

 

Arhel Nisole

 Nathalie ARHEL and Sébastien NISOLE

 

 IRIM - UMR 9004

1919, route de Mende, 34293 Montpellier

 

Phone: +33 4 34 35 94 53, +33 4 34 35 94 54

Email: nathalie.arhel@irim.cnrs.fr  sebastien.nisole@irim.cnrs.fr

       

 

Website

 

 

Our team is interested in the mechanisms that control host-virus interactions at the level of the infected cell. Viruses rely on cellular transport machineries to travel from their site of entry to their site of replication, assembly and egress, with speed and efficiency to avoid rapid overpowering by the cell’s innate immunity and degradation machineries. We study how viruses use these cellular transport machineries for intracellular trafficking, including nuclear import for viruses that replicate in the nucleus such as HIV, and conversely how the cell responds to viral infections by initiating efficient intrinsic and innate immunities. In particular, we seek to understand the cellular pathways that mediate host-innate immune response to viral infection, focusing on the molecular mechanisms triggering interferon response following viral infections. Our aims are to (i) identify the determinants of viral movement within cells, (ii) characterize the mediators of the antiviral activity of interferon, (iii) determine the role of post-translational modifications in the control of viral infections.


 

Keywords: HIV trafficking, nuclear import, restriction factors, innate immunity, interfero.

 

 

Main publications : 

 

Portilho DM, Fernandez J, Ringeard M, Machado AK, Boulay A, Mayer M, Müller-Trutwin M, Beignon AS, Kirchhoff F, Nisole S, Arhel NJ. (2016). Endogenous TRIM5α Function Is Regulated by SUMOylation and Nuclear Sequestration for Efficient Innate Sensing in Dendritic Cells. Cell Rep. 14(2):355-69.

Dutrieux J, Maarifi G, Portilho DM, Arhel NJ, Chelbi-Alix MK, Nisole S. (2015). PML/TRIM19-Dependent Inhibition of Retroviral Reverse-Transcription by Daxx. PLoS Pathog. 11(11):e1005280.

Fernandez J, Portilho DM, Danckaert A, Munier S, Becker A, Roux P, Zambo A, Shorte S, Jacob Y, Vidalain PO, Charneau P, Clavel F, Arhel NJ. (2015). Microtubule-associated proteins 1 (MAP1) promote human immunodeficiency virus type I (HIV-1) intracytoplasmic routing to the nucleus. J Biol Chem. 290(8):4631-46.

Dutrieux J, Portilho DM, Arhel NJ, Hazan U, Nisole S. (2015). TRIM5α is a SUMO substrate. Retrovirology. 12:28.

Maarifi G, Chelbi-Alix MK, Nisole S. (2014). PML control of cytokine signaling. Cytokine Growth Factor Rev. 25(5):551-61.

Lelek M, Di Nunzio F, Henriques R, Charneau P, Arhel NJ, Zimmer C. (2012). Superresolution imaging of HIV in infected cells with FlAsH-PALM. Proc Natl Acad Sci U S A. 109(22):8564-9.

Carthagena L, Bergamaschi A, Luna JM, David A, Uchil PD, Margottin-Goguet F, Mothes W, Hazan U, Transy C, Pancino G, Nisole S. (2009). Human TRIM gene expression in response to interferons. PLoS One. 4(3):e4894.

Arhel NJ, Nisole S, Carthagena L, Coutant F, Souque P, Brussel A, Estaquier J, Charneau P. (2008). Lack of endogenous TRIM5alpha-mediated restriction in rhesus macaque dendritic cells. Blood. 112(9):3772-6.

Arhel NJ, Souquere-Besse S, Munier S, Souque P, Guadagnini S, Rutherford S, Prévost MC, Allen TD, Charneau P. (2007). HIV-1 DNA Flap formation promotes uncoating of the pre-integration complex at the nuclear pore. EMBO J. 26(12):3025-37.

Arhel NJ, Genovesio A, Kim KA, Miko S, Perret E, Olivo-Marin JC, Shorte S, Charneau P. (2006). Quantitative four-dimensional tracking of cytoplasmic and nuclear HIV-1 complexes. Nat Methods. 3(10):817-24.

Nisole S, Stoye JP, Saïb A. (2005). TRIM family proteins: retroviral restriction and antiviral defence. Nat Rev Microbiol. 3(10):799-808. Review.

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