Parent Category: Laboratoires Published: Friday, 17 February 2012

Autophagy and Infections

 

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 Martine BIARD-PIECHACZYK

 

 IRIM - UMR 9004

 1919, route de Mende, 34293 Montpellier

 

Phone: +33 4 34 35 94 55

Email : martine.biard@irim.cnrs.fr

 

Website

 

 

 

The team was the first to describe that HIV envelope-mediated apoptosis of the uninfected CD4 T cells, a key feature of HIV-1 pathogenesis, depends on autophagy. The main objective is now to study the relationship between HIV-1 and autophagy and can be delineated into two main parts:

- Mechanisms triggered in the uninfected CD4 T cells inducing autophagy, and then apoptosis. These data will be of great importance to understand the mechanisms leading to immunodeficiency during HIV-1 infection.

- Role of autophagy during HIV-1 replication and mechanisms by which HIV-1 modulates this process in infected cells. We are studying the interactions that exist between autophagic and HIV-1 proteins in both HIV-1-infected CD4 T cells and macrophages. The aim of this axe is to determine the strategies developed by HIV-1 to hijack autophagy for its own replication and thus to provide new insights into therapeutic strategies for the future.

 

Keywords: Autophagy, HIV-1, Apoptosis, Innate immunity

 

 

Main publications

  • D. J. Klionsky, ..., M. Biard-Piechaczyk, ...L. Espert, ... Guidelines for the Use and Interpretation of Assays for Monitoring Autophagy. Autophagy, 2012, In press.
  • M. Biard-Piechaczyk, L. Espert, S. Borel, G. De Bettignies, O. Coux. HIV-1, Ubiquitin and Ubiquitin-like proteins: the dialectic interactions of a virus with a sophisticated network of post-translational modifications. Biol. Cell. 2012, 104, 1–24
  • I. Pombo-Grégoire, S. Borel, C. Richetta, L. Meyniel, F. Pradezynski, O. Diaz, A. Deloire, M. Le Breton, O. Azocar, P.E. Mangeot, V. Navratil, P.E. Joubert, M. Flacher, P.O. Vidalain, P. André, V. Lotteau, M. Biard-Piechaczyk, C. Rabourdin-Combe, M. Faure. IRGM is a common target of RNA viruses that subvert the autophagy network. PLoS Pathog. 2011, 7(12): e1002422
  • L. Espert, M. Varbanov, V. Robert-Hebmann, S. Sagnier, I. Robbins, F. Sanchez, V. Lafont, M. Biard-Piechaczyk. Differential role of autophagy in CD4 T cells and macrophages during X4 and R5 HIV-1 infection. Plos One. 2009. 4 (6) e5787.
  • M. Denizot, M. Varbanov, L. Espert, V. Robert-Hebmann, S. Sagnier, E. Garcia, M. Curriu , R. Mamoun, J. Blanco, M. Biard-Piechaczyk. HIV-1 gp41 fusogenic function triggers autophagy in uninfected cells. Autophagy. 2008. 4:998-1008.
  • M. Grimaldi, L. Molina, V. Robert-Hebmann, L. Espert, B. Gay, C. Devaux, C. Granier, M. Biard-Piechaczyk. Proteomic analysis of the cellular responses induced in uninfected cells by cell-expressed X4 HIV-1 envelope. Implication of the mitochondria. Proteomics. 2007. 7: 3116-30.
  • L. Espert, M. Denizot, M. Grimaldi, V. Robert-Hebmann, B. Gay, M. Varbanov, P. Codogno, M. Biard-Piechaczyk. Autophagy is involved in T cell death after binding of HIV-1 envelope proteins to CXCR4. J. Clin. Invest. 2006, 116 : 2161-2172.
  • - Cet article a été sélectionné dans la base « Faculty of 1000 »
  • - Cet article a fait l'objet de la couverture du journal (Aout 2006)
  • - Cet article a fait l'objet d'un commentaire par B. Levine and D.L. Sodora. HIV and CXCR4 in a kiss of autophagic death. J. Clin. Invest. 2006, 116 : 2078-80.
  • - Cet article a fait l'objet d'un commentaire dans la section « In Brief », Nature Reviews Microbiology. 2006, 4 :724.
  • - Cet article a fait l'objet d'un commentaire dans la section « Research Highlights », Nature Medecine. 2006, Sept; 12, 1003.
  • B. Ahr, M. Denizot, V. Robert-Hebmann, A. Brelot, M. Biard-Piechaczyk. Identification of the cytoplasmic domains of CXCR4 involved in Jak2 and STAT3 phosphorylation. J. Biol. Chem. 2005, 280 :6692-6700.
  • J. Roland, B.J. Murphy, B. Ahr, V. Robert-Hebmann, V. Delauzun, K.E. Nye, C. Devaux , M. Biard-Piechaczyk. Role of the intracellular domains of CXCR4 in SDF-1-mediated signaling. Blood. 2003, 101:399-406.
  • R. Roggero, V. Robert-Hebmann, S. Harrington, J. Roland, L. Vergne, S. Jaleco, C. Devaux, M. Biard-Piechaczyk. Binding of human immunodeficiency virus type 1 gp120 to CXCR4 induces mitochondrial transmembrane depolarization and cytochrome c-mediated apoptosis independently of Fas signaling. J. Virol, 2001, 75:7637-7650.

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